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. 2015 Nov 3;6:310. doi: 10.3389/fphys.2015.00310

Figure 1.

Figure 1

Dexamethasone (Dex) treatment increases AQP2 protein abundance in rat IMCD. (A) Rat IMCD suspensions were exposed to 0.1 μM Dex for 6 h and lysed in RIPA buffer. Equal amounts of supernatant were immunoblotted with antibodies to AQP2 and GAPDH. (B) Rat IMCD suspensions were exposed to 0.1 μM AVP, 10 μM forskolin (FSK) or 0.1 μM Dex. Equal amounts of lysates were immunoblotted with antibodies to AQP2, UT-A1, and GAPDH. Western blot signals were quantified by NIH ImageJ (compared to control, *P < 0.05, **P < 0.01, n = 3).