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. 2015 Nov 3;6:310. doi: 10.3389/fphys.2015.00310

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Copyright © 2015 Chen, Cai, Klein, Laur and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dexamethasone increases AQP2 expression in the transfected HEK 293 cell. (A) HEK293 cells were grown in 6-well plates and transfected with pcDNA3-AQP2 1.0 μg for 48 h. After incubation with 0.1 μM Dex for 6 h, cells were collected and solubilized in RIPA buffer. Equal amounts of proteins in the supernatant were analyzed by immunoblotting with AQP2 and GAPDH antibodies (n = 4). (B) Time course experiment. HEK293 cells were transfected with pcDNA3-AQP2 1.0 μg for 48 h then treated by 0.1 μM Dex for the indicated time. AQP2 protein expression was analyzed by immunoblotting with AQP2 antibody. Western blot signals were quantified by NIH ImageJ (compared to control, ^**P < 0.01, n = 4).