Figure 3.

Dexamethasone increases AQP2 cell membrane expression. HEK293 cells were grown in 6-well plates and transfected with pcDNA3-AQP2 1.0 μg for 48 h. After incubation with Dex or 0.1 μM RU486 pre-treatment 30 min + Dex for 6 h, cells were processed for cell membrane protein biotinylation and lysed in RIPA buffer. The biotinylated proteins were collected by streptavidin beads. Cell membrane AQP2 was analyzed by immunoblotting with AQP2 antibody. Western blot signals were quantified by NIH ImageJ (compared to control, ^**P < 0.01, n = 4).