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Effect of dexamethasone on AQP2 stability. HEK293 cells were transfected with pcDNA3-AQP2. After 48 h, the cells were pre-treated with CHX (100 μg/ml) for 30 min, then treated without or with dexamethasone (0.1 μM) for the indicated time. Total AQP2 protein was analyzed by western blot. Protein signals were quantified by NIH ImageJ (compared to CHX, *P < 0.05, **P < 0.01, n = 3).
