Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Nov 3;9:435. doi: 10.3389/fncel.2015.00435

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Miller, Liu and Puthanveettil.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Using the LKMTA and DTA to measure the reduction in fast mitochondrial transport in a single pair of pre-drug/post-nocodazole movies. (A) A set of images from a single well, before and after application of nocodazole. (B) The absolute velocity maps for the control and nocodazole treated samples at a single time point. The bar to the side shows the scaling between pixel intensity and local velocity. (C) The velocity distributions for control and nocodazole treated samples generated from the absolute velocity maps. (D) The same data plotted on a log scale. (E) A pictorial representation of the graph in (D), where pixel intensity is equal to the number of pixels. The bar to the side shows the scaling. (F) The same data again, but plotted on a log-log graph. (G) A pictorial representation of the data in (F). (H) A maximum Z-projection of all of the absolute velocity maps in the time series. (I) A maximum Z-projection of all of the tracks produced by the Mass Particle Tracker plugin in the time series. (J) Overlay of the output of the LKMTA and Mass Particle Tracker algorithm in red and blue. There is a similar overall reduction in the flow maps and the number of tracks, as well as a correspondence between their distributions; bar = 25 μm.