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. 2015 Nov 3;9:435. doi: 10.3389/fncel.2015.00435

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Copyright © 2015 Miller, Liu and Puthanveettil.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Automated removal of spurious motion using the LKMTA. (A) Illustration of the steps used to normalize the velocity histograms produced by the LKMTA. (B) Illustration of the steps used to remove transient changes in the velocity profiles caused by stage drift and illumination flicker. (C) The plots of the velocity histograms of the pre-drug and post-nocodazole conditions. (D) The velocity profiles over the velocity range characteristic of fast mitochondrial transport. (E) The ratio of movements with velocities in the range of 0.25–4 μm/s for the control and nocodazole treated samples (ave ± sd).