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. 2015 Oct 20;2015:720457. doi: 10.1155/2015/720457

Figure 6.

Figure 6

ROS scavenger inhibited the activation of NLRP3 inflammasome in AMs exposed to burn serum. AMs were challenged with burn serum with NAC, a ROS scavenger, for 24 h or 48 h. ROS were examined by way of FACS (a) and were quantified by way of mean fluorescence intensity (b). The activation of NLRP3 ((c) and (d)) and Caspase-1 ((e) and (f)) was measured by immunoblots or real-time PCR. The release of IL-1β (g) and IL-18 (h) in the supernatant was tested by ELISA. Results represent mean ± SEM of six independent experiments. ∗ P < 0.05 compared to the value at SS group. # P < 0.05 compared to the value at BS group. SS, sham serum; BS, burn serum; AM, alveolar macrophage.