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. 2015 Sep 30;43(3):184–194. doi: 10.5941/MYCO.2015.43.3.184

Table 1. Effect of carbon, nitrogen, concentration of nitrogen source and carbon to nitrogen ratio on the mycelia growth of Calocybe indica [16].

graphic file with name mb-43-184-i001.jpg

aCarbon source: Six synthetic carbon sources namely, glucose (control), starch, sucrose, lactose, xylose, fructose were used. Their equivalent quantities were replaced with glucose of the Maltova broth (glucose, 10.0; Maltova, 10.0; NaNO3, 1.5; KH2PO4, 0.5; MgSO4 · 7H2O, 0.2; KCl, 0.1; FeSO4 · 7H2O, 0.02). All ingredients are given in g/L.

bNitrogen source: Four different nitrogen sources replaced sodium nitrate (control) of maltova broth on the basis of equivalent quantities.

cNitrogen concentration: Six concentrations of yeast extract (YE) and sodium nitrate (best N sources), i.e., 0.05%, 0.1%, 0.15%, 0.20%, 0.25%, and 0.30% were tried using Maltova broth with 1% xylose.

dC : N ratio: the medium was prepared with different C : N ratios, 6 : 1 to 14 : 1, using yeast extract at 2.5 g (best concentration from previous experiment) and different amounts of xylose.