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. 2015 Nov 3;6:953. doi: 10.3389/fpls.2015.00953

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Copyright © 2015 Ye, Li, Wang, Wang, Li, Du, Zhang and Shou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fe deficiency activates MPK3 and MPK6. (A) Transcription levels of MPK3 and MPK6 in the leaf and root of WT seedlings under Fe-sufficient (50 μM EDTA-Fe) and -deficient (0 μM EDTA-Fe) conditions. All values are expressed relative to the expression level under Fe-sufficient conditions (control—set to 1.0) as appropriate. Data are shown as the mean ±SEM (n = 3). (B) Immunoprecipitation kinase assay of MPK3 and MPK6 activity using Anti-p44/42 MAPK antibody. Ten-day-old seedlings were transferred to Fe-sufficient or -deficient nutrient solutions. Column marked with “**” indicate a highly significantly difference (P < 0.01).