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. 2015 Nov 3;6:953. doi: 10.3389/fpls.2015.00953

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Copyright © 2015 Ye, Li, Wang, Wang, Li, Du, Zhang and Shou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ethylene production and expression of ACS2 and ACS6 in mpk3 and mpk6 mutants. (A) Ethylene production in mpk3 and mpk6 mutants. (B) Transcript abundances of ACS2 and ACS6 genes in mpk3 and mpk6 mutants. Ten-day-old seedlings were transferred to Fe-sufficient (50 μM EDTA-Fe) or -deficient (0 μM EDTA-Fe) nutrient solutions. Ethylene accumulated over a 24 h period from 10 seedlings in the headspace of GC vials was measured at 4 days after the treatment. qRT-PCR was conducted using RNA extracted from whole seedlings at 7 days after –Fe treatment. Data are shown as the mean ±SEM (n = 3). Columns marked with “^*” indicate a significant difference (P < 0.05), and “^**” indicate a highly significantly difference (P < 0.01).