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. 2015 Nov 3;5:15986. doi: 10.1038/srep15986

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(A) Anchorage -independent growth was measured by colony formation in soft agar. Colonies with >50 cells were scored. (B) DNA damage assay. Cells were treated with MMS. The amount of damaged DNA lesions was detected using ARP. (C) MMS induced apoptosis was analyzed by flow cytometry. (D) Cellular sensitivity was determined by growth inhibition experiments after cells were treated with MMS.