Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Nov 3;5:15978. doi: 10.1038/srep15978

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Recorded by GCaMP3 ( ), GCaMP6f () and GCaMP3_fast () are: (a) spontaneous whole-cell cytosolic Ca²⁺ signals in Ad-CMV-virus infected cells imaged confocally at 120 Hz (n ≥ 10); (b) expanded beats, marked by stars; (c) representative traces of Ca²⁺ release after application of 3 mM caffeine (n ≥ 9), imaged confocally at 30 Hz; (d) average values of 70% Ca²⁺ rise time in spontaneously beating cardiac myocytes; (e) average values of 70% Ca²⁺ decay time in spontaneously beating cardiac myocytes; (f) average values of 70% relaxation time after caffeine application; (g) Ca²⁺ current activated Ca²⁺ release (CICR) in voltage clamped neonatal cardiomyocytes (n ≥ 7), imaged confocally at 120 Hz. Cells are voltage clamped at −50 mV and depolarized to 0 mV; (h) average values of depolarization-activated 70% Ca²⁺ rise time; (i) average values of depolarization-activated 70% relaxation time.

Inline graphic — Recorded by GCaMP3 ( ), GCaMP6f () and GCaMP3_fast () are: (a) spontaneous whole-cell cytosolic Ca²⁺ signals in Ad-CMV-virus infected cells imaged confocally at 120 Hz (n ≥ 10); (b) expanded beats, marked by stars; (c) representative traces of Ca²⁺ release after application of 3 mM caffeine (n ≥ 9), imaged confocally at 30 Hz; (d) average values of 70% Ca²⁺ rise time in spontaneously beating cardiac myocytes; (e) average values of 70% Ca²⁺ decay time in spontaneously beating cardiac myocytes; (f) average values of 70% relaxation time after caffeine application; (g) Ca²⁺ current activated Ca²⁺ release (CICR) in voltage clamped neonatal cardiomyocytes (n ≥ 7), imaged confocally at 120 Hz. Cells are voltage clamped at −50 mV and depolarized to 0 mV; (h) average values of depolarization-activated 70% Ca²⁺ rise time; (i) average values of depolarization-activated 70% relaxation time.