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. 2015 Nov 3;5:16053. doi: 10.1038/srep16053

Figure 2. IL-17 promotes NSCLC angiogenesis via STAT3 activation.

Figure 2

(a) mRNA expression of IL-17R in NSCLC cell lines. (b) Western blotting showed that phosphorylation of STAT3 were obviously increased as early as 6 h after IL-17 treatment and lasted for 24 h after IL-17 stimulation. A549 cells were incubated with IL-17 at the indicated concentrations for 24 h or at 100 ng/ml for the indicated time. (c) Immunofluorescence assays showed that recombinant human IL-17(100 ng/ml for 24 h) significantly elevated the expression of p-STAT3 in A549 cells. Photomicrographs were taken at ×200 magnification. Control, PBS. (d) A549 cells or A549-siRNA-STAT3 cells were treated with IL-17 at 100 ng/ml for 24 h, media was harvested, added to HUVECs plated on Matrigel. HUVEC were seeded in 96-well plates coated with matrigel and treated with CCM or CCM-IL-17 from A549 cells or A549-siRNA-STAT3 cells for 16 h. In this experiment, HUVEC incubated with 0.5% serum containing 1640 media served as a negative control. Tubular structures were photographed at 40× magnification and tube length was measured as described in ‘Materials and Methods’. Tube length data is presented as mean ± standard deviation of three samples for each treatment.