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. 2015 Nov 3;5:16017. doi: 10.1038/srep16017

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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A protein microarray approach was used to characterize the angiogenesis-related growth factors in BM Soup (a) and deactivated BM Soup (b), while normal saline control (c) was used as a negative control. A cytokine microarray was also used to profile cytokines in the BM Soup (e), deactivated BM Soup (f) and normal saline control (g,d,h) after background subtraction and removal of overlaps in normal saline control, quantification of factors (intensity) was shown. Several angiogenesis-related proteins and two cytokines were identified in BM soup. After the deactivation process using proteinase K + 95 °C, all these factors and cytokines were below the detection level of the two protein arrays. All data were mean ± S.D. from three independent experiments. All data were normalized to the intensity of positive control (PC, three pairs of dots at the corners).