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| Viral proteins(amino acid) | P0 | P5 | P9 | P12 | P17 |
|---|---|---|---|---|---|
| HA (424) | Asp | Gly | — | Gly | Gly |
| M1 (240) | Asn | — | Thr | Thr | Thr |
| M2 (14) | Asn | — | Ser | Ser | Ser |
| NP (294) | Lys | — | — | — | Asn |
| PA (609) | Val | — | — | — | Ile |
Note: P0, parent virus B/Florida/04/2006; P5, 5th mouse passage; P9, 9th mouse passage; P12, 12th mouse passage; and MA, mouse-adapted virus after 17 passages.
