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. 2015 Nov 3;6:960. doi: 10.3389/fpls.2015.00960

FIGURE 6.

FIGURE 6

GhABI5 responds to ABA, inhibiting seed germination and inducing downstream gene expression. (A) Transgenic plants overexpressing GhABI5 are hypersensitive to ABA. Seeds of abi5-3(abi5), Col, GhABI5 complemented lines (1, 2, 3#) and overexpression lines (9, 11, 12#) were sown on filter paper with different concentrations of ABA without stratification, and photographed after 2 days. (B) Germination rate of transgenic seeds (35S:GhABI5/abi5-1# and 35S:GhABI5/Col-9#), abi5 and Col. Without ABA, the germination rate is similar among the lines, compared with the different rates resulting from 0.5 μM ABA treatment. A seed with open green cotyledons is considered germinated. (C) GhABI5 positively regulates the expression levels of ABA-responsive genes. The freshly harvested seeds, including complemented transgenic seeds (1, 2, 3#), abi5 and Col, were used for qRT- PCR. Bars represent the SE of three biological replicates. (∗P < 0.05; ∗∗P < 0.01).