Figure 4.

Regulation of MUTYH in renal fibrosis in vivo, as shown in UUO kidneys. Immunohistochemical staining of MUTYH showed granular accumulation in tubules of UUO kidneys (a). Both intensity (left panel) and the number of positive tubules (right panel) increased in obstructed kidneys for 3 days and then declined by 10 days ((b), P < 0.001). Western blotting showed MUTYH expression in kidneys with dominant mitochondrial bands of ~57 kDa (c). When α-SMA (red) was observed ((d), upper panel), MUTYH staining (in green) localized to the cytoplasm, without overlapping DAPI (in blue). Otherwise, when α-SMA was highly expressed, a few cells with nuclear MUTYH staining (white arrows) were detected in fibrotic interstitial cells ((d), low panel).