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. Author manuscript; available in PMC: 2016 Feb 17.

Published in final edited form as: Biochemistry. 2015 Feb 3;54(6):1364–1370. doi: 10.1021/bi5014722

Table 2.

The pK_a of all oligos in 30 mM sodium cacodylate ± 40% PEG-300. The ΔpK_a are also shown to emphasize the difference upon adding 40% PEG-300.

	pK_a No PEG	pK_a 40% PEG	ΔpK_a
T1	6.67 ± 0.01	7.03 ± 0.05	0.36
Mod1T3	6.66 ± 0.01	7.04 ± 0.05	0.38
Mod1T5	6.25 ± 0.04	6.71 ± 0.12	0.46
Mod1T10	6.14 ± 0.02	6.54 ± 0.05	0.40
Mod1T15	nd.^*	nd.^*	nd.^*
Mod1T20	nd.^*	nd.^*	nd.^*
Mod2T3	6.66 ± 0.03	7.16 ± 0.05	0.50
Mod2T5	6.68 ± 0.04	7.14 ± 0.05	0.46
Mod2T10	6.62 ± 0.02	6.98 ± 0.07	0.36
Mod2T15	6.33 ± 0.02	6.71 ± 0.06	0.38
Mod2T20	5.98 ± 0.05	6.48 ± 0.06	0.50
Mod3T3	6.70 ± 0.03	7.00 ± 0.06	0.30
Mod3T5	6.27 ± 0.02	6.63 ± 0.08	0.36
Mod3T10	6.12 ± 0.02	6.42 ± 0.05	0.30
Mod3T15	nd.^*	nd.^*	nd.^*
Mod3T20	nd.^*	nd.^*	nd.^*

^*

T_m of oligo ≤ 20 °C, thus pK_a not determined at 20 °C.