Table 3.
ESTs from adult cDNAs with predicted amino-terminal signal peptides and with homologs in C. elegans
| Cluster | Score | P | Conventional cDNAs | Oligo-capped cDNAs | Wormpep ID | SignalP criteria | SignalP scores | Signal in C. elegans? | Description of C. elegans gene | |||
| C-p | Amino acids | SP-p | SP? | |||||||||
| (a) Signal peptides predicted in both N. brasiliensis and C. elegans | ||||||||||||
| NBC00012 | 86 | 6e-18 | 4 | 0 | CE20223 | YYYYS | 0.533 | 16 | 1.000 | Y | Y | Unknown (similar to NBC00237) |
| NBC00031 | 80 | 3e-16 | 2 | 2 | CE17924 | YYYYS | 0.932 | 18 | 0.999 | Y | Y | Unknown |
| NBC00237 | 84 | 5e-17 | 1 | 2 | CE20223 | YYYYS | 0.671 | 19 | 1.000 | Y | Y | Unknown (similar to NBC00012) |
| NBC00258 | 145 | 1e-35 | 1 | 0 | CE00133 | YYYYS | 0.524 | 19 | 0.999 | Y | Y | FAR-1 fatty acid/retinol-binding protein |
| NBC00266 | 129 | 6e-31 | 1 | 0 | CE19630 | YYYYS | 0.662 | 20 | 1.000 | Y | Y | Unknown |
| NBC00314 | 147 | 3e-36 | 1 | 1 | CE03639 | YYYYS | 0.708 | 19 | 0.987 | Y | Y | Transthyretin-like family |
| NBC00327 | 94 | 2e-20 | 1 | 0 | CE00906 | YYYYS | 0.542 | 25 | 0.998 | Y | Y | Unknown |
| NBC00336 | 138 | 2e-33 | 1 | 0 | CE23545 | YYYYS | 0.903 | 17 | 1.000 | Y | Y | Unknown |
| NBC00354 | 91 | 4e-21 | 4 | 0 | CE16530 | YYYYS | 0.511 | 17 | 0.943 | Y | Y | Unknown |
| NBC00472 | 215 | 8e-57 | 1 | 0 | CE04886 | YYYYS | 0.319 | 15 | 0.999 | Y | Y | Signal sequence receptor |
| NBC00487 | 55 | 7e-09 | 1 | 0 | CE05972 | YYYYS | 0.979 | 21 | 0.988 | Y | Y | Unknown |
| NBC00495 | 51 | 3e-07 | 1 | 1 | CE13171 | YYYYS | 0.566 | 19 | 0.999 | Y | Y | Transthyretin-like family |
| NBC00502 | 176 | 3e-45 | 1 | 0 | CE32298 | YYYYS | 0.634 | 20 | 1.000 | Y | Y | Ectonucleotide pyrophosphatase/phosphodiesterase |
| NBC00592 | 80 | 1e-15 | 0 | 3 | CE17924 | YYYYS | 0.920 | 16 | 1.000 | Y | Y | Unknown |
| NBC00606 | 81 | 4e-16 | 0 | 2 | CE02454 | YYYYS | 0.399 | 20 | 1.000 | Y | Y | Similar to O. volvulus hypodermal antigen Ov-17 |
| NBC00615 | 207 | 3e-54 | 0 | 1 | CE04533 | YYYYS | 0.995 | 18 | 1.000 | Y | Y | LBP-1 fatty acid-binding protein |
| NBC00616 | 61 | 3e-10 | 0 | 1 | CE20257 | YYYYS | 0.754 | 19 | 0.993 | Y | Y | Unknown |
| NBC00633 | 153 | 4e-38 | 0 | 1 | CE03639 | YYYYS | 0.450 | 17 | 1.000 | Y | Y | Transthyretin-like family |
| NBC00641 | 145 | 1e -35 | 0 | 1 | CE33289 | YYYYS | 0.219 | 19 | 0.930 | Y | Y | Unknown |
| NBC00643 | 102 | 2e-22 | 0 | 2 | CE27850 | YYYYS | 0.961 | 17 | 0.999 | Y | Y | Unknown |
| NBC00706 | 50 | 9e-07 | 0 | 1 | CE06014 | YYYYS | 0.466 | 20 | 1.000 | Y | Y | Unknown |
| NBC00720 | 12 | 3e-30 | 0 | 1 | CE16958 | YYYYS | 0.967 | 19 | 0.998 | Y | Y | NLP-13 neuropeptide |
| NBC00742 | 60 | 3e-10 | 0 | 1 | CE16731 | YYYYS | 0.880 | 21 | 0.993 | Y | Y | Unknown |
| NBC00748 | 50 | 4e-07 | 0 | 1 | CE02932 | YYYYS | 0.804 | 17 | 0.998 | Y | Y | Transthyretin-like family |
| NBC00767 | 79 | 7e-16 | 0 | 1 | CE31662 | YYYYS | 0.559 | 17 | 1.000 | Y | Y | Unknown |
| (b) Signal peptides predicted in N. brasiliensis but not C. elegans | ||||||||||||
| NBC00028 | 104 | 1e-23 | 1 | 1 | CE00431 | YYYYS | 0.731 | 18 | 0.999 | Y | N | Globin |
| NBC00124 | 128 | 8e-31 | 1 | 1 | CE00431 | YYYYS | 0.731 | 18 | 0.999 | Y | N | Globin |
| NBC00144 | 195 | 7e-51 | 1 | 0 | CE29663 | YYNYS | 0.866 | 19 | 0.963 | Y | N | Transport-secretion protein |
| NBC00197 | 143 | 8e-35 | 3 | 6 | CE00431 | YYYYS | 0.557 | 16 | 1.000 | Y | N | Globin |
| NBC00272 | 144 | 2e-35 | 1 | 0 | CE32475 | YYNYS | 0.262 | 22 | 0.513 | Y | N | Unknown |
| NBC00328 | 147 | 4e-36 | 3 | 4 | CE00431 | YYYYS | 0.523 | 17 | 0.999 | Y | N | Globin |
| NBC00581 | 122 | 7e-29 | 0 | 1 | CE00431 | YYYYS | 0.404 | 21 | 0.998 | Y | N | Globin |
| NBC00601 | 93 | 5e-20 | 0 | 1 | CE30218 | YYYYS | 0.535 | 34 | 0.944 | Y | N | Unknown |
| NBC00607 | 159 | 4e-40 | 0 | 1 | CE29597 | YYNYS | 0.529 | 18 | 0.786 | Y | N | Unknown |
Entries in table do not match numbers in Figure 2, which includes predicted signal anchors. SignalP criteria are C-score (raw cleavage site score); S-score (signal peptide score); Y-score (combined cleavage site score); mean S score; and assignation as signal peptide (S as in all entries above; otherwise A for signal anchor or N for neither). SignalP scores are as follows: C-p: probability of predicted cleavage site being correct; amino acids: length of predicted signal peptide in amino acids; SP-p: probability of existence of signal peptide; SP?: overall prediction for signal peptide. Note that NBC00028 is almost identical to the cuticular globin of N. brasiliensis (P51536), and NBC00197 and NBC00328 are closely related, whereas NBC0124 and NBC00581 are more similar to, but not identical to, the body-wall form of globin (P51535).