Table 2.
Effect of cartilage injury on mRNA abundance relative to Gapdh of ECM genes in separate tissue pools from WT mice.
| Acan | Vcan V1 | Vcan V2 | Col1a1 | Col2a1 | Col3a1 | |
|---|---|---|---|---|---|---|
| Naive | ||||||
| Cartilage and subchondral bone | 1.87 | 26.9 | ND | 1720 | 1.22 | 1.6 |
| Meniscus and synovium | 0.85 | 5.21 | 0.16 | 1280 | 0.15 | 24.6 |
| Patellar Tendon | 0.04 | 6.33 | 0.05 | 962 | ND | 2.6 |
| 1 week | ||||||
| Cartilage and subchondral bone | 10.1 (5.4)† |
12.7 (0.5) |
0.47 (−) |
3550 (2.1) |
1.87 (1.5) |
62.9 (39 |
| Meniscus and synovium | 61.8 (72.3) |
15.2 (2.9) |
15.2 (92) |
10300 (8.0) |
0.38 (2.6) |
4010 (163) |
| Patellar Tendon | 130 (3386) |
11.1 (1.8) |
17.2 (366) |
10900 (11) |
0.42 (−) |
4451 (1734) |
| 4 weeks | ||||||
| Cartilage and subchondral bone | 7.97 (4.3) |
8.85 (0.3) |
0.18 (−) |
4880 (2.8) |
1.38 (1.1) |
54.8 (34) |
| Meniscus and synovium | 8.12 (9.5) |
2.80 (0.5) |
1.75 (10.6) |
2770 (2.2) |
0.80 (5.4) |
1080 (44) |
| Patellar Tendon | 83.7 (2180) |
6.87 (1.1) |
4.41 (94) |
7380 (7.7) |
17.7 (−) |
3200 (1245) |
†
Italicized numbers in parentheses are fold change relative to naïve levels. ND = not detected (Ct > 35), with no fold change calculation possible ( – ).