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. Author manuscript; available in PMC: 2015 Nov 3.
Published in final edited form as: Biochemistry. 2015 Mar 6;54(10):1859–1862. doi: 10.1021/acs.biochem.5b00119

Figure 2.

Figure 2

Mutational frequency of 8-oxodGuo (OG) and Fapy•dG (FG) in HEK293T cells also transfected with NC siRNA or siRNA for pol λ knockdown. HEK293T cells were plated in six-well plates at 25% confluence. After a 24-h incubation period, they were transfected with appropriate siRNA (NC or pol λ) and Lipofectamine. One day before the transfection of the plasmid, cells were seeded in 24-well plates at 50% confluence. Cells were then co-transfected with another aliquot of siRNA and either the control or the lesion-containing plasmid. After a 24-h incubation, progeny plasmids were isolated and analyzed as described.25 The data represent the average of two independent experiments (shown in Table S2 in the SI). Others include semitargeted mutations near the lesion as indicated in Table S2 in SI.