Table 1.

Primers used for amplification of B. melitensis omp25, omp28 and omp31 genes with vector cohesive overhangs (bold)

No	Primer name	Sequence (5′-3′)	Length	Expected product bp
1.	B. melitensis omp25 F	GACGACGACAAGATGCGCACTCTTAAGTCTCT	32	668
2.	B. melitensis omp25 R	GAGGAGAAGCCCGGTTAGAACTTGTAGCCGAT	32
3.	B. melitensis omp28 F	GACGACGACAAGATGAACACTCGTGCTAGCAATT	34	779
4.	B. melitensis omp28 R	GAGGAGAAGCCCGGTTACTTGATTTCAAAAACGA	34
5.	B. melitensis omp31 F	GACGACGACAAGATGAAATCCGTAATTTTGGCGT	34	749
6.	B. melitensis omp31 R	GAGGAGAAGCCCGGTTAGAACTTGTAGTTCAGAC	34