Table 3.

Comparable analysis between the serotype-specific RT-PCR assays and the CDC DENV1-4 RT-PCR

		CDC DENV1 RT-PCR
		negative	Positive
DENV1 RT-PCR	negative	36	0
	positive	6	43
		CDC DENV2 RT-PCR
		negative	positive
DENV2 RT-PCR	negative	67	0
	positive	6	12
		CDC DENV3 RT-PCR
		negative	positive
DENV3 RT-PCR	negative	76	0
	positive	2	7
		CDC DENV4 RT-PCR
		negative	positive
DENV4 RT-PCR	negative	78	0
	positive	1	6

A sample was classified as positive in the CDC DENV-1-4 real-time RT-PCR assays if the amplification curve crossed the threshold line within 37 cycles

Five of the six DENV1 RT-PCR positive samples, two of the six DENV2 RT-PCR positive samples, both the DENV3 RT-PCR positive samples, and the DENV4 RT-PCR positive sample that tested negative in respective CDC DENV-1-4 RT-PCR singleplex assay crossed the threshold line between 37 and 40 cycles in the CDC assays