Fig. 6.

Impact of extracellular fractions of E. coli on cell culturability of E. coli at 45 °C. Effect of high temperature on growth of E. coli SUBE01: a impact on cell turbidity b and influence on the formation of CFUs, upon supplementation with the extracellular fractions of E. coli SUBE01 as organic supplement and 20 mM MgSO₄ with 5 mM EDTA as inorganic supplement and untreated cells were referred as control. Cells were grown in minimal agar at 45 °C up to 72 h and after 10 h of growth organic supplement and inorganic supplement were added. At every 12 h interval, cells were diluted and grown in minimal agar at 37 °C for 24 h. No significant changes were observed at 45 °C (a, b), when cells were grown with the extracellular fractions of E. coli SUBE01. All experiments were carried out three times and the standard deviations for all data have been indicated by error bars