Table 1. List of capsid amino acid residues and their scores from entropy and ConSurf analysis.

Amino acids are arranged according to their order in the P1 sequence. The predicted amino acid residues are mostly located close to known epitopes except for residue VP2-191 that is 5 aa apart from VP2-196. nr, No reference antigenic site reported; GHL, VP1 G-H loop.

P1 position	Viral protein (VP)	VP position	Entropy value	ConSurf score	Epitope reported previously	Residue located on external surface
159	2	74	1.1	3.985	nr	Yes
163	2	78	1.156	4.458	nr	Yes
171	2	86	1.183	4.472	nr	Yes
173	2	88	0.867	3.644	nr	Yes
218	2	133	1.37	4.662	nr	Yes
219	2	134	1.704	4.802	134	Yes
276	2	191*	1.627	4.789	nr	Yes
292	2	207	1.018	3.65	nr	No
338	3	35	0.988	3.523	nr	No
373	3	70	1.031	4.541	70	Yes
374	3	71	1.348	5.29	71	Yes
434	3	131*	0.931	3.074	nr	Yes
523	3	220*	1.25	5.034	nr	Yes
566	1	42	1.096	2.529	nr	Yes
567	1	43*	1.222	2.996	nr	Yes
568	1	44*	1.42	3.555	nr	Yes
569	1	45*	1.554	3.861	nr	Yes
623	1	99	1.585	4.287	nr	Yes
625	1	101	1.078	2.38	nr	Yes
663	1	139	1.177	2.855	139	GHL
666	1	142	1.185	2.915	142	GHL
673	1	149	1.263	3.132	149	GHL
721	1	197	1.484	3.783	nr	Yes
722	1	198	1.343	3.3	198	Yes

^*Residues selected for site-directed mutagenesis study.