Fig. 3. Endocytic uptake of IFITM3 proteins. Live A549 cells expressing HA-tagged IFITM3 from human, pig or microbat (or untransduced cells) were incubated either on ice (a) or at 37 °C (b) with FITC-conjugated anti-HA and Alexa Fluor 546-conjugated transferrin. After cell fixation, nuclei were stained with DAPI and the coverslips examined by confocal microscopy. The two right-hand columns showed merged images and phase-contrast images. Bar, 10 µm.
