Fig. 6. Microbat and pig IFITM3 inhibit cell entry mediated by lyssavirus G proteins. A549 cells stably expressing GFP, human IFITM3, pig IFITM3 (clone 1) or microbat IFITM3 (clone 2) were infected with pseudotyped viruses that expressed the envelope glycoprotein from Mokola virus (MOKV), West Caucasian bat virus (WCBV), Lagos bat virus (LBV) or rabies virus strain Evelyn Rokitniki Abelseth (ERA). At 48 h post-infection, luciferase reporter activity was measured and normalized to GFP-expressing cells (relative infectivity of 1 corresponding to 25 000–80 000 relative light units according to pseudotype used). Results are shown as means±sd (n = 3) and data are representative of three independent experiments.