SUV39H2 mRNA expression is upregulated in ALL. (A) SUV39H2 mRNA levels in PBMNCs obtained from four healthy donors, primary blasts from 30 patients with ALL, as well as ALL cell lines (B-ALL, n = 9 and T-ALL, n = 8) as measured by RT-PCR. (B) SUV39H2 mRNA levels in paired samples obtained at diagnosis and at remission from patients with ALL (n = 30) as measured by RT-PCR. (C) Comparison of SUV39H2 and H3K9me3 protein expression in PBMNCs obtained from four healthy donors with that in ALL cell lines (B-ALL, n = 3 and T-ALL, n = 4) by Western blot analyses. mRNA levels were normalized to B2M expression levels. P values were calculated using Student’s t test (***P < .001).