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. 2015 Oct 28;8(5):368–375. doi: 10.1016/j.tranon.2015.07.003

Figure 4.

Figure 4

Effect of SUV39H2 overexpression on proliferation and chemosensitivity. (A) SUV39H2 mRNA expression and (B) protein expression of HA-SUV39H2 and H3K9m3 in Jurkat cells following lentiviral transduction. (C) Densitometry of H3K9me3 protein band obtained from triplicate experiments. (D) Comparison of proliferation between control Jurkat EV and Jurkat SUV39H2 OE. (E) Cell viability in Jurkat EV and Jurkat SUV39H2 OE cells following treatment with increasing concentrations of cytarabine. (F) Representative figure of Annexin V and PI staining and (G) graphical quantification of average Annexin V and PI staining results in Jurkat EV and Jurkat SUV39H2 OE cells after treatment with 100 nM cytarabine. Data are presented as mean ± SE; P values were calculated using Student’s t test (*P < .05; ** P < .01; ***P < .001).