Table 1.
Top 30 Upregulated and Downregulated Probes in Affymetrix Expression Array Analysis
| Top 30 upregulated probes in NHUC-BMI1 relative to isogenic NHUC-vector | ||||||
|---|---|---|---|---|---|---|
| Donor 1 |
Donor 2 |
Average |
||||
| Probeset ID | Gene Symbol | Fold Change | P Value | Fold Change | P Value | Fold Change |
| 202237_at | NNMT | 176.00 | 4.48E-05 | 24.92 | 2.19E-03 | 100.46 |
| 202238_s_at | NNMT | 51.50 | 9.67E-06 | 21.41 | 8.08E-04 | 36.46 |
| 209949_at | NCF2 | 4.30 | 3.51E-02 | 24.66 | 1.64E-03 | 14.48 |
| 210809_s_at | POSTN | 3.08 | 1.30E-01 | 25.74 | 3.76E-02 | 14.41 |
| 1560679_at | LOC151438 | 21.13 | 5.02E-03 | 3.30 | 3.89E-01 | 12.22 |
| 225911_at | NPNT | 9.85 | 2.32E-04 | 13.28 | 1.58E-03 | 11.56 |
| 209351_at | KRT14 | 14.65 | 5.71E-03 | 7.83 | 5.77E-02 | 11.24 |
| 201141_at | GPNMB | 2.05 | 4.97E-01 | 19.30 | 7.32E-02 | 10.68 |
| 219630_at | PDZK1IP1 | 14.47 | 3.31E-05 | 6.83 | 1.35E-02 | 10.65 |
| 206912_at | FOXE1 | 12.95 | 1.69E-05 | 6.84 | 4.51E-04 | 9.89 |
| 232861_at | PDP2 | 9.89 | 1.77E-04 | 9.12 | 3.35E-04 | 9.51 |
| 220014_at | PRR16 | 13.05 | 4.97E-05 | 5.76 | 2.78E-02 | 9.41 |
| 224823_at | MYLK | 6.94 | 3.05E-03 | 11.64 | 9.66E-04 | 9.29 |
| 244780_at | SGPP2 | 2.80 | 1.11E-01 | 14.98 | 5.54E-02 | 8.89 |
| 231331_at | – | 11.28 | 2.45E-05 | 5.34 | 7.21E-04 | 8.31 |
| 202766_s_at | FBN1 | 2.91 | 6.49E-03 | 13.49 | 7.69E-04 | 8.20 |
| 1553589_a_at | PDZK1IP1 | 10.11 | 3.39E-04 | 6.09 | 8.71E-03 | 8.10 |
| 1556499_s_at | COL1A1 | 5.76 | 1.56E-03 | 10.06 | 2.03E-03 | 7.91 |
| 214701_s_at | FN1 | 12.13 | 1.22E-03 | 3.65 | 5.85E-02 | 7.89 |
| 228854_at | – | 3.43 | 1.77E-01 | 11.77 | 3.31E-02 | 7.60 |
| 219911_s_at | SLCO4A1 | 8.13 | 1.27E-03 | 6.36 | 2.92E-03 | 7.25 |
| 213040_s_at | NPTXR | 4.06 | 1.57E-02 | 9.89 | 6.24E-04 | 6.98 |
| 236044_at | PPAPDC1A | 5.46 | 5.68E-03 | 8.32 | 3.46E-04 | 6.89 |
| 211478_s_at | DPP4 | 4.93 | 6.20E-04 | 8.53 | 2.31E-03 | 6.73 |
| 226388_at | TCEA3 | 6.40 | 4.22E-03 | 6.88 | 9.70E-04 | 6.64 |
| 205547_s_at | TAGLN | 2.34 | 2.51E-01 | 10.62 | 1.73E-02 | 6.48 |
| 226281_at | DNER | 2.12 | 3.72E-02 | 10.68 | 9.26E-03 | 6.40 |
| 213385_at | CHN2 | 5.16 | 3.75E-03 | 7.62 | 4.38E-03 | 6.39 |
| AFFX-DapX-5_at | – | 7.40 | 4.89E-04 | 5.33 | 1.21E-03 | 6.37 |
| 1555724_s_at | TAGLN | 2.18 | 2.51E-01 | 9.85 | 1.09E-02 | 6.01 |
| Top 30 downregulated probes in NHUC-BMI1 relative to isogenic NHUC-vector | ||||||
| Donor 1 | Donor 2 | Average | ||||
| Probeset ID |
Gene Symbol | Fold Change | P Value | Fold Change | P Value | Fold Change |
| 233011_at | ANXA1 | − 19.86 | 5.33E-03 | − 47.91 | 5.49E-04 | − 33.88 |
| 202265_at | BMI1⁎ | − 26.36 | 1.79E-05 | − 41.21 | 2.76E-06 | − 33.79⁎ |
| 244026_at | – | − 42.42 | 1.47E-04 | − 34.65 | 1.84E-04 | − 38.54 |
| 232113_at | – | − 18.18 | 6.90E-03 | − 31.34 | 1.13E-03 | − 24.76 |
| 232541_at | – | − 21.04 | 2.31E-04 | − 30.72 | 2.24E-04 | − 25.88 |
| 224567_x_at | MALAT1 | − 25.83 | 1.55E-03 | − 25.61 | 2.05E-03 | − 25.72 |
| 226675_s_at | MALAT1 | − 34.50 | 1.01E-03 | − 24.15 | 1.68E-03 | − 29.33 |
| 1558678_s_at | MALAT1 | − 39.70 | 2.94E-04 | − 23.55 | 1.16E-03 | − 31.62 |
| 240815_at | – | − 13.81 | 4.12E-05 | − 21.98 | 2.29E-04 | − 17.90 |
| 209550_at | NDN | − 4.62 | 5.67E-04 | − 21.65 | 4.71E-05 | − 13.13 |
| 227062_at | NCRNA00084 | − 19.22 | 8.02E-03 | − 21.58 | 1.97E-02 | − 20.40 |
| 219795_at | SLC6A14 | − 5.89 | 2.17E-02 | − 20.82 | 4.19E-06 | − 13.36 |
| 224559_at | MALAT1 | − 20.19 | 3.59E-04 | − 20.51 | 2.38E-03 | − 20.35 |
| 235592_at | – | − 11.66 | 1.44E-03 | − 20.30 | 1.02E-05 | − 15.98 |
| 240038_at | – | − 16.43 | 2.64E-05 | − 20.01 | 2.65E-05 | − 18.22 |
| 205513_at | TCN1 | − 17.30 | 1.34E-03 | − 19.98 | 7.03E-05 | − 18.64 |
| 217523_at | CD44 | − 14.96 | 1.19E-03 | − 19.86 | 4.83E-04 | − 17.41 |
| 236879_at | – | − 23.07 | 8.36E-06 | − 19.48 | 2.27E-05 | − 21.28 |
| 236545_at | – | − 9.68 | 8.53E-04 | − 19.12 | 6.65E-05 | − 14.40 |
| 233300_at | – | − 12.36 | 4.85E-04 | − 18.34 | 5.70E-05 | − 15.35 |
| 220940_at | ANKRD36B | − 14.52 | 6.40E-06 | − 17.71 | 3.15E-06 | − 16.12 |
| 231929_at | IKZF2 | − 11.76 | 7.49E-03 | − 17.55 | 8.09E-05 | − 14.66 |
| 242476_at | – | − 12.15 | 2.13E-05 | − 17.37 | 9.94E-05 | − 14.76 |
| 230746_s_at | – | − 2.17 | 2.12E-01 | − 16.38 | 7.62E-03 | − 9.28 |
| 228483_s_at | TAF9B | − 3.51 | 2.93E-03 | − 16.08 | 1.27E-03 | − 9.79 |
| 244741_s_at | MGC9913 | − 4.71 | 5.82E-04 | − 15.78 | 3.08E-04 | − 10.24 |
| 236322_at | – | − 22.66 | 4.14E-03 | − 15.54 | 1.15E-03 | − 19.10 |
| 230229_at | DLG1 | − 11.57 | 2.47E-04 | − 15.47 | 1.38E-04 | − 13.52 |
| 235879_at | MBNL1 | − 15.59 | 2.12E-05 | − 15.08 | 1.58E-04 | − 15.33 |
| 235138_at | – | − 17.81 | 1.69E-04 | − 14.98 | 2.68E-04 | − 16.39 |
Fold change is expression in NHUC-BMI1 relative to the isogenic, empty vector transduced NHUC.
⁎
Endogenous BMI1 was downregulated in NHUC-BMI1. Retroviral-transduced BMI1 was not detected by the probe because it is located in the 3’ UTR not expressed from the retroviral vector.