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. Author manuscript; available in PMC: 2015 Nov 3.
Published in final edited form as: Nat Med. 2015 Jun 15;21(7):815–819. doi: 10.1038/nm.3887

Figure 3.

Figure 3

PAD4 deficiency facilitates wound repair in normoglycemic mice. (a) Images of H&E staining (upper panels) and confocal microscopy (lower panels) of wounds from WT and Padi4−/− mice 3 days after injury. Scale, 50 ~m. Presence of extracellular DNA (blue streaks) in the scab of WT mice are indicated by yellow arrows, while intact neutrophils in that of Padi4−/− mice are indicated by yellow arrowheads in the H&E images. (b) Representative Western blots of wounds from WT (+/+) and Padi4−/− (−/−) mice. See Supplementary Fig. 8 for quantifications. (c) Photographs of healing wounds of WT and Padi4−/− mice up to 7 days after wounding. Scale, 5 mm. (d) Changes in wound area compared to day 0. Per order in the bar chart, n = 16, 16, 15, 12 for WT groups, n = 12, 12, 12, 9 for Padi4−/− groups, *P<0.05, **P<0.01, ***P<0.001 versus WT, Student’s t test. (e) Percent of WT and Padi4−/− mice that completed wound healing on day 13 and 14 after injury. Day 13: WT (2/11) vs Padi4−/− (6/9), P=0.065; Day 14: WT (4/16) vs Padi4−/− (10/13), **P<0.01; two-tailed Fisher's exact test. (f) Re-epithelialization as determined from H&E staining on wounds from WT and Padi4−/− mice 3 days post wounding. See Supplementary Fig. 9 for histology of wounds. n = 9 for WT, n = 6 for Padi4−/−, ***P<0.001, Student’s t test.