Figure 2.

Functional properties of the FNTB G > C polymorphism rs116238662. A) Structure of the FNTB gene locus. Black boxes represent exons, they are not drawn to scale. The 5' and 3'UTR are highlighted in white. Positions of FNTB polymorphisms are indicated together with minor allele frequencies given in brackets. B) In silico transcription factor binding sites, rs11623866 alleles and the surrounding sequence are shown. Transcription factor names and their putative binding sites are shown above and below the corresponding FNTB alleles, respectively. C) Genotype-dependent binding of nuclear proteins to the human FNTB promoter as analyzed by EMSA. Arrow indicates specifically retarded bands observed upon addition of nuclear extracts. A nuclear protein bound the C allele exclusively. D) Genotype-dependent activity of FNTB 5'-regulatory region reporter constructs expressed in A2780 and SKOV3 ovarian carcinoma cells. E) Relative expression of FNTB mRNA in leukocytes of healthy donors, renal cancer and ovarian cancer tissues dependent on rs11623866 genotypes