Figure EV4.

Localization of Msn2-GFP

1. The ratio between the nuclear and the cytoplasmic Msn2-GFP over time after the exposure to stress is shown. In the Δmsn5 strain, we observe high nuclear localization of Msn2-GFP at the beginning of the experiment, compared to WT.
2. Msn2-GFP nuclear localization shows a stress-specific prototypical profile. The plot compares the dynamics of Msn2-GFP localization in KCl and diamide stress conditions. The average dynamics profile of all the examined strains in two independent repeats is shown.
3. Msn2-GFP nuclear localization is correlated between stress conditions. The area under the curve (AUC) of Msn2-GFP localization dynamics was quantified for each mutant and stress condition (Materials and Methods). Scatter plot of the AUC values in KCl and diamide for all strains is shown.
4. Two biological repeats of localization experiment lead to similar AUC values. Scatter plot of the AUC estimated for different strains after the exposure to KCl in two independent experiments.