Figure EV3. DISSECT generates single-cell suspensions with comparable yield to the conventional disaggregation method.

1. Whole field immunofluorescence image of single-cell suspensions post-disaggregation. The conventional sample was imaged prior to methanol permeabilization. Autofluorescence was used to image the cell body.
2. Phase-contrast image of single-cell suspensions.
3. Quantitative comparison of yields between different disaggregation conditions starting from the same amount of intestinal material from the same animal. Error bars represent SEM from n = 4 technical replicates. Two independent repeats were performed on different animals.