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. Author manuscript; available in PMC: 2015 Nov 3.
Published in final edited form as: J Immunol. 1997 Mar 1;158(5):2477–2489.

FIGURE 3.

FIGURE 3

Nucleotide sequences of the VHDJH junctions (CDR3-FR4 areas) of the mAbs produced by B-1a, B-1b, and B-2 cells including unencoded nucleotides. The nucleotide sequences of the relevant portions of the germ-line D genes are given for comparison, and appear above the expressed mAb D segments. Dashes indicate identity with germline D segment sequences. Asterisks indicate the reverse complement of the known germline D segment. Unencoded nucleotide additions are between VH and D, D and D, and D and JH gene segments. The TGG triplet encoding the invariant Trp that marks the beginning of the 3′ conserved portion of the JH gene is listed under FR4. These sequences are available from EMBL/GenBank/DDBJ under the accession numbers of the respective VH genes as listed in Table I.

* Denotes a D gene that is utilized in an inverted position.