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. 2015 Nov 3;10(11):e0141994. doi: 10.1371/journal.pone.0141994

Fig 3. Induction of cold-seeking behaviors by carvacrol was not detected in the two-plate temperature assay.

Fig 3

(A) Schematic of the experiment. CD-1 mice were intravenously injected with vehicle or carvacrol at 50 mg/kg. 10 min after injection, mice were introduced for 3 min into the temperature-preference apparatus, where they were allowed to move freely and select between two plates set to 30°C (left plate) and 10°C right plate). (B) The percentage of time spent on each plate was measured. This time reveals the preference for the temperature of the respective plate. N = 8 mice per group. P>0.05, t-test. (C) Core temperature was measured during the described protocol every 5 min by wireless temperature transmitters. Carvacrol injection, but not vehicle injection, induced hypothermia. * P<0.05, 2-way Repeated measures ANOVA followed by Student-Neuman-Keuls post-hoc test.