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a, G-band staining showing a normal karyotype for all the lines analyzed. b, After in vitro spontaneous differentiation of control and C9 carrier iPSC lines, cells were staining for α-fetoprotein (AFP, endoderm), desmin (mesoderm), βIII-tubulin (ectoderm), and Hoechst (nuclei). All lines showed differentiation towards derivates of three germ layers. Scale bars: 20 mm.
