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. 2015 Oct 23;8:3067–3077. doi: 10.2147/OTT.S84888

Figure 3.

Figure 3

Construction of the PVAX1-HPV58 mE6E7FcGB plasmid and expression of the target proteins assessed by immunofluorescence staining.

Notes: (A) Diagram of the construction of the recombinant DNA vaccine PVAX1-HPV58 mE6E7FcGB. HPV58 mE6E7 was cloned into the plasmid PCI-sig-Fc-GPI, and the fused cassette sig-HPV58 mE6E7-Fc-GPI was subsequently excised from PCI-sig-HPV58 mE6E7-Fc-GPI plasmid and inserted into the upstream of IRES in PVAX1-IRES-GM/B7.1 vector to create PVAX1-HPV58 mE6E7FcGB. (B) The transfected 293T cells were concurrently incubated with a rabbit anti-human IgG-FITC antibody (green) and a mouse anti-human B7-1-PE antibody (red). Immunofluorescence revealed the expression of sig-HPV58 mE6E7-Fc-GPI (a, green, ×50) and GM-CSF/B7.1 (b, red, ×50) at the cells membrane.

Abbreviations: IgG-FITC, immunoglobulin G–conjugated fluorescein isothiocyanate; IRES, internal ribosome entry site; HPV, human papillomavirus.