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. 2015 Oct 31;71(Pt 11):2267–2277. doi: 10.1107/S1399004715016429

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© Chen et al. 2015

This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

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(a) Sequence alignment of S. enterica AadA with a representative set of homologues from different bacteria retrieved from a BLAST search (UniProt accession numbers are given in parentheses): Salmonella bongori (F8VG00), Providencia stuartii (I0E075), Myxococcus xanthus (Q1DBM4), Escherichia coli (C4NV14), Serratia odorifera (D4E6F0), Variovorax paradoxus (C5CZ95), Pseudomonas mendocina (A4XUC2) and Enterococcus faecalis D6 (C7UVI9). Strictly conserved residues are highlighted in red and conservative substitutions are in red font. The secondary structure of S. enterica AadA is shown above the alignment. The residues subjected to mutagenesis are indicated with asterisks above the alignment. (b) Electrostatic surface potential of AadA. The colour spectrum ranges from deep red (−7kT) to deep blue (+7kT). Orientations are as in Fig. 2 ▸(a). (c) Surface conservation mapped by ConSurf (Celniker et al., 2013 ▸; Ashkenazy et al., 2010 ▸). The colour spectrum ranges from magenta (highest conservation) to cyan (lowest conservation). Orientations are as in Fig. 2 ▸(a).