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. 2015 Oct 31;71(Pt 11):2267–2277. doi: 10.1107/S1399004715016429

Table 2. Minimum inhibitory concentrations (MICs) of aminoglycoside antibiotics for strains with wild-type or mutant AadA.

nd, not determined.

    MIC (gml1)
Strain aadA genotype/amino-acid substitution Streptomycin Spectinomycin Kanamycin Tobramycin Amikacin Gentamicin
DA6192 Wild type 128 192 6 3 4 0.75
DA18900 aadA::cat 4 24 6 3 4 0.75
DA29580 E87A 4 24 6 nd nd nd
DA29582 E87Q 4 24 6 nd nd nd
DA29584 W112A 16 24 6 nd nd nd
DA29586 W112F 48 24 6 nd nd nd
DA29588 D182A 32 48 6 nd nd nd
DA29590 D182N 24 48 6 nd nd nd
DA29592 R192A 6 24 6 nd nd nd
DA29594 K205A 4 24 6 nd nd nd

MICs were determined with E-tests (bioMrieux). All culture media were M9 plus 0.2% glycerol.

The cat cassette replaced the entire aadA gene.