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Behavior of 5mC and its oxi-mCs in bisulfite conversion and the following PCR step
| Base | Bisulfite conversion | BS Sequencing result |
|---|---|---|
| 5mC | 5mC | C |
| 5hmC | CMS | C |
| 5gmC | 5gmC | C |
| 5fC | U | T |
| 5caC | U | T |
| C | U | T |
Abbreviations: CMS: cytosine methylenesulfonate; 5gmC: β-glucosyl-5-hydroxymethylcytosine
