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Time-course studies in human aortic endothelial cells (HAEC) exposed to summer 2006 (sum06) ambient particulate matter (APM) by quantitative real-time RT-PCR. Induction of CYP1A1, PTGS2, TIPARP, and CCL-2 gene expression in HAEC exposed to sum06 APM for 30 min, 1 h, 2 h, 3 h, and 4 h compared with control cells treated with H2O. Sum06 APM-induced gene expression was significantly increased after 1 h, 2 h, 3 h, and 4 h. n = 3, *P ≤ 0.005, #P ≤ 0.05.
