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| Method | Hanging-drop vapor diffusion |
| Plate type | EasyXtal 15-well |
| Temperature (K) | 293 |
| Protein concentration (mgml1) | 20 |
| Buffer composition of protein solution | 50mM Na2HPO4/NaH2PO4 pH 7.4, 150mM NaCl |
| Composition of reservoir solution | 0.1M HEPES pH 7.0, 18%(w/v) PEG 4000, 0.1M sodium acetate, 0.1M lithium sulfate |
| Composition of droplet | 2l (protein) + 3l (reservoir) |
| Volume of reservoir (ml) | 1.0 |
