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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Am J Transplant. 2015 Feb 19;15(5):1241–1252. doi: 10.1111/ajt.13098

Figure 2. Islets preparations are separated by the anatomic distribution of portal venous blood flow.

Figure 2

A. PBMC flow cytometry confirming loss of Gal expression in GTKO animals.

B. Immunohistochemical staining of galactose-alpha-1,3-galactose was analyzed to determine adequate segregation of islets by hemiliver. At both 1 hour (p=0.05) and 24 hours (p=0.01) staining between islet phenotypes was significantly different. There was no difference in the positivity of staining from 1 to 24 hours within each phenotype (WT p=0.97, GTKO p=0.96).

C. Representative Image of α-Gal positive WT islet cluster at 24 hours.

D. Representative image of α-Gal negative GTKO islet cluster at 24 hrs.