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. Author manuscript; available in PMC: 2016 Nov 1.

Published in final edited form as: Photochem Photobiol. 2015 Oct 12;91(6):1444–1451. doi: 10.1111/php.12521

A) Quantitative IHC using MIA of caspase 3 cleavage in CTCL cell lines following conventional ALA-PDT. Cells were collected at various time points post treatment, data for each cell line are shown as follows: untreated control, 3, 6, 12, 24 and 48 hours post PDT. *p < 0.01 as compared to caspase 3 activation in HH, SZ4 and SeAx cells. B) PpIX fluorescence (red) in prototypical FAS-high (a) and FAS-low (b) CTCL cell lines after incubation with ALA, c and d show respective untreated controls. C) The histogram shows quantitative MIA-based analysis of intracellular PpIX in Hut78 and HH cells before (− ALA) and after (+ ALA) incubation with 1mM ALA for 6 hours (*p < 0.01 as compared to untreated controls). All images and data shown in this as well as in the subsequent figures are representative of three separate experiments.

A) Quantitative IHC using MIA of caspase 3 cleavage in CTCL cell lines following conventional ALA-PDT. Cells were collected at various time points post treatment, data for each cell line are shown as follows: untreated control, 3, 6, 12, 24 and 48 hours post PDT. *p < 0.01 as compared to caspase 3 activation in HH, SZ4 and SeAx cells. B) PpIX fluorescence (red) in prototypical FAS-high (a) and FAS-low (b) CTCL cell lines after incubation with ALA, c and d show respective untreated controls. C) The histogram shows quantitative MIA-based analysis of intracellular PpIX in Hut78 and HH cells before (− ALA) and after (+ ALA) incubation with 1mM ALA for 6 hours (*p < 0.01 as compared to untreated controls). All images and data shown in this as well as in the subsequent figures are representative of three separate experiments.