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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Anal Biochem. 2015 Sep 3;491:43–51. doi: 10.1016/j.ab.2015.08.027

Table 2.

Mass and calculated molar extinction coefficients at 280 nm and 25 °C for BSA, HEWL, and ovalbumin [44-47].

Protein Literature ε280
(g−1 L cm−1)
Mass ε280 in PBS
(g−1 L cm−1) ± 95% CI
Mass ε280 in water
(g−1 L cm−1) ± 95% CI
Molar ε280 in PBS
(mM−1 cm−1) ± 95% CI
BSA 0.667 [44] 0.61 ± 0.06 0.61 ± 0.01 40 ± 4
HEWL 2.64 [45-46] 2.26 ± 0.17* 2.56 ± 0.14* 32.3 ± 2.4
Ovalbumin 0.69 – 0.76 [47] 0.63 ± 0.03 0.63 ± 0.02 26 ± 1
*

p = 0.004