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. 2015 Oct 1;142(19):3429–3439. doi: 10.1242/dev.121384

Fig. 6.

Fig. 6.

Disrupting PCP patterning leads to structural defects in basal body polarity. (A) Apical intercellular region of a single MCC labeled with CLAMP-GFP and Centrin-RFP, which when combined display the orientation of cilia with respect to the direction of ciliary beating (CLAMP points in the opposite direction). (B-L) Plots displaying the mean basal body orientation and associated mean vector length of MCCs under various experimental conditions. Each arrow represents a measure of the orientations of a single MCC, with increased length correlated with less variation between basal bodies within each MCC. Dark and light arrowheads represent data from separate experimental repeats, with each experiment involving measures from at least five cells from at least three embryos each. The combined data are displayed for control (B, n=2113 orientations, 31 cells), Pk2-MO#1 knockdown (C, n=1556 orientations, 32 cells), Pk2-MO#2 knockdown (D, n=1057 orientations, 18 cells), Pk2 overexpression (E, n=2426 orientations, 34 cells), Pk2-ΔPET overexpression (F, n=3122 orientations, 35 cells), Pk2-ΔPETΔLIM overexpression (G, n=1941 orientations, 34 cells), Pk2-ΔC2 overexpression (H, n=2275 orientations, 33 cells), Vangl1 overexpression (I, n=2843 orientations, 35 cells), Dvl1 overexpression (J, n=2086 orientations, 37 cells), Dvl1-ΔPDZpartial overexpression (K, n=2797 orientations, 36 cells) and Dvl2-ΔPDZpartial overexpression (L, n=2177 orientations, 32 cells) conditions. (M) The mean resultant vector length from the combined measurements for all MCCs across all embryos from both experimental treatments, serving as an overall metric for PCP disruption. (N-P) Stills from the end of movies showing the traces of beads carried by flow across the epidermis of St.31 X. laevis embryos that were uninjected (N) or were injected with either Pk2-MO (O) or a high dose of vangl1 mRNA (P). (Q) Quantification of the flow rate from five beads traced in the movies of which the final frame is shown in N-P. Error bars indicate s.e.m. Scale bars: 1 mm.