Fig. 1.

Prdm12 expression in Xenopus, chicken and amphioxus embryos. (A-G) Whole-mount ISH analysis of prdm12 expression in Xenopus. In C,E,F, transverse sections at the levels indicated in B,D are shown. (H-M) Double ISH comparing prdm12 with the indicated genes. In panels H,L and M, both Prdm12 and the second probe are revealed in dark blue. In panels I-K, prdm12 is in dark blue and the second probe is in light blue. (N) Diagram summarizing the expression domains of Prdm12 and of HD TFs in the ventricular zone of the Xenopus ventral caudal neural tube. (O) A transverse section of the neural tube of a chick embryo hybridized for Prdm12 and immunostained for Dbx1 (red). (P,Q) Prdm12 and Engrailed are co-expressed in restricted cells of the ventral nerve cord (arrows) of amphioxus larvae (48 hpf). Lateral and dorsal views as well as a transverse section are shown.