Fig. 2.

prdm12 is dependent on RA signaling and Pax6, and is repressed by Dbx1 and Nkx6. (A) RT-PCR analysis of the expression of the indicated genes in animal caps isolated from wild-type or noggin-injected (100 pg/blastomere) embryos, cultivated in the presence or absence of RA. Expression levels were compared with the expression level of uninjected caps, which was defined as 1. (B,C,F,G) prdm12 expression in Xenopus neurula-stage embryos injected unilaterally at the 2-cell stage with the indicated mRNA (500 pg/blastomere). (D,E,H,K). prdm12 is downregulated in the spinal cord of E12.5 pax6^Sey/Sey mutants and is expanded in dbx1^lacZ/lacZ and nkx6-1^−/− embryos (brackets) (n≥2 for all mutant lines). IS, injected side.