Skip to main content
. 2015 Oct 1;142(19):3394–3402. doi: 10.1242/dev.122341

Fig. 5.

Fig. 5.

A 31-amino acid domain in Rbp4 is required for binding to Fest. (A) Diagram of Rbp4 protein structure and truncated Rbp4 proteins tested in S2 cells for binding to Fest. RRM, RNA recognition motif. (B) Anti-HA, anti-Myc western blot of anti-Myc immunoprecipitations (top panels) and 10% input (bottom panels) from S2 cells transfected with HA-Fest alone or HA-Fest with various Myc-Rbp4 truncations, as indicated. (C) Alignment of Rbp4 residues 245-275 with Rbp4 homologs in other species. (D,E) Anti-GFP immunostaining of Rbp4-eYFP/+ testis (D) and Rbp4-eYFP/+; fest testis (E). Scale bar: 100 µm in E for D and E. (F) Biotin RNA pull-down from Rbp4-eYFP/+, fest testis, using the wild-type 130 nt cycB 3′ UTR probe.